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KMID : 0355519920160010051
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Korean Journal of Oral Biology
1992 Volume.16 No. 1 p.51 ~ p.56
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Whole-genomic DNA Probes and Restriction Analysis for the Identification of Mutans Streptococci
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Choe Se-Won
Lee Jeong-Sook
Choe Seon-Jin
Kim Kack-Kyun
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Abstract
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DNA hybridization with whole-genomic DNA probe and restriction pattern analysis were done in search for a useful method for identification of the species of mutans group streptocci. Dot blots of genomic DNA of five strains (serotypes a, b, c and g) of mutans streptococci and one strain of Streptococcus sanguis were hybridized with 32P-labeled whole-genomic DNA of Streptococcus mutans strain 10449 (serotype c) as a probe. Also, genomic DNAs of each strain were digested with two restriction enzymes (EcoRI and HindIII) and the restriction patterns were compared on 0.7% agarose gel after electrophoresis. Whole-genomic DNA probe of Strep. Mutans strain 10449 was able to detect 3ng of homologous DNA perdot and was also able to detect the same amount of genomic DNA of a different strain of the same serotype with about the same intensity as shown on the autoradiogram. Genomic DNAs of different serotypes of mutans streptococci and of Strep. Sanguis strain MPC1 were hybridized weakly with the probe at 50-100ng per dot. Genomic DNAs of six bacteria of two different genera(Bacteroides gingivalis and intermedius) were not hybridized with the probe at any concentration used in experiment. Restriction-digested patterns of genomic DNAs were all different from strain to strain, indicating different genomic structures between each serotypes of mutans streptococci.
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KEYWORD
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DNA probes, periodontal disease, Bacteroides spp, diagnosis
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